Gambogic Acid Inhibits Gastric Cancer Cell Proliferation through Necroptosis

Figure 4

Necroptosis inhibitors inhibited GA-induced cell death. Indicated inhibitors were used to treat cells to inhibit necroptosis at a different step. Knockdown of PGAM5 was used to attenuate PGAM5 activity. (a) PGAM5 expression was checked by western blot in PGAM5 siRNA transfected cells. (b) Inhibitors were used to treat AGS cells for 24 hours and the phosphorylation of Drp-1 was examined by western blot in treated cells and PGAM5 siRNA transfected cells. (c) Annexin V-FITC/PI double staining of AGS cells treated with GA and indicated inhibitors or PGAM5 knockdown to detect cell death. (d) Inhibitors were used to treat AGS cells for 24 hours and the phosphorylation of Drp-1 was examined by western blot in treated cells and PGAM5 siRNA transfected cells. (e) Annexin V-FITC/PI double staining of AGS cells treated with GA and indicated inhibitors or PGAM5 knockdown to detect cell death. ; .

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