Research Article

Circulation of Dengue Virus Serotypes in the City of Makkah, Saudi Arabia, as Determined by Reverse Transcription Polymerase Chain Reaction

Table 2

Dengue virus specific primers and their expected product size.

SerotypePrimer pairSize of PCR products (bp)

First step: RT-PCR
 All serotypesD1 + D2511
Second step: serotype detection
 DENV-1D1 + TS1482
 DENV-2D1 + TS2119
 DENV-3D1 + TS3290
 DENV-4D1 + DEN4392