Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR

Table 1

LPDs and LPCs of the serial dilutions for norovirus GI/GII, C. jejuni, and E. histolytica native and card samples.

Pathogen	Conventional sample			Stool card sample
	Sample 1	Sample 2	Sample 3	Sample 1	Sample 2	Sample 3
Norovirus GI
Norovirus GII
C. jejuni	1 : 8	1 : 4	1 : 8	1 : 8	1 : 8	1 : 8
	12.500/mL	25.000/mL	12.500/mL	12.500/mL	12.500/mL	12.500/mL
E. histolytica	1 : 16	1 : 8	1 : 16	1 : 16	1 : 16	1 : 16
	344/mL	688/mL	344/mL	344/mL	344/mL	344/mL

LPCs are stated as calculated pathogen concentration (organisms/mL). steps retested by Microgen real-time PCR. Ct of norovirus (provided by reference laboratory): GI sample 1, Ct 20; GI sample 2, Ct 20; GI sample 3, Ct 18; GII sample 1, Ct 17; GII sample 2, Ct 18; GII sample 3, Ct 18.