Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR

Table 2

Results of the storage trial for C. jejuni, E. histolytica, and norovirus GI/II.

	Duplicate	Day 0	Day 7/14/21	Day 28	Day 35/42	Detection; rates
C. jejuni Samples 1, 2	1	pos	pos	pos	pos	97.6%
	2	pos	pos	pos	pos
C. jejuni Sample 3	1	pos	pos	neg	pos
	2	pos	pos	pos	pos
E. histolytica Samples 1, 2, 3	1	pos	pos	pos	pos	100%
	2	pos	pos	pos	pos
Norovirus GI Sample 1	1	pos	pos	pos	pos	97.6%
		Crypto.	Crypto.	Crypto.	Crypto.
	2	neg	pos	pos	pos
			Crypto.	Crypto.	Crypto.
Norovirus GI Samples 2, 3	1	pos	pos	pos	pos
	2	pos	pos	pos	pos
Norovirus GII Samples 1, 2, 3	1	pos	pos	pos	pos	100%
	2	pos	pos	pos	pos

pos, positive; neg, negative; Crypto, Cryptosporidium spp.
content of samples: C. jejuni: 100.000 organisms/mL and E. histolytica: 5500 organisms/mL; Ct of norovirus (provided by reference laboratory): GI sample 1, Ct 20; GI sample 2, Ct 22; GI sample 3, Ct 23; GII sample 1, Ct 11; GII sample 2, Ct 16; GII sample 3, Ct 19.