Development of IgY-Based Sandwich ELISA as a Robust Tool for Rapid Detection and Discrimination of Toxigenic Vibrio cholerae

Figure 1

Binding capability evaluation of α-OmpW and α-CtxB antibodies. (a) Strip 1: protein size marker; Strip 2: purified IgY from control group chicken showed no band with recombinant OmpW; and Strip 3: the band of 27 kDa recombinant OmpW reacted with α-OmpW antibodies. (b) Strip 1: protein size marker; Strip 2: purified IgY from control group chicken showed no band with recombinant CtxB; and Strip 3: the band of 17 kDa recombinant CtxB reacted with α-CtxB antibodies.