Research Article
The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance
Table 1
Bacterial strains and plasmids.
| | Strains or plasmids | Relevant characteristic (s) |
| | Strains | | | E. coli JM109 | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 (argF-lacZYA) U169 80dlacZ | | PA01 | Reference strain; genome completely sequenced | | PA01ΔampG | ampG (PA4393) deleted PA0114 |
| | Plasmids | | | pMD18-ampG1-35 | pMD18 vectors carrying ampGs of 35 wild type P. aeruginosa strains, respectively (this work) | | pMD18-ampGPA | pMD18 vector carrying ampG of PA01 (this work) | | pUCP24 | pUC18-derived broad-host-range vector; Gmr | | pUCP24-ampG1-35 | ampG genes from pMD18-ampG1-35 cloned into pUCP24, respectively; Gmr (this work) | | pUCP24-ampGPA | ampG gene of pMD18-ampGPA cloned into pUCP24; Gmr (this work) | | pUCP24-ampGmut | pUCP24 vector carrying site-directedly mutated ampG of PA01 (this work) | | pUCP24-ampGPA-I53S | | | pUCP24-ampGPA-I53T | | | pUCP24-ampGPA-W90R | | | pUCP24-ampGPA-W90S | |
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