Research Article
The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance
Table 2
Primers used in this work.
| | Primer | Sequence | Purpose |
| | PAampG-F | 5′GGGATCCCAACGCGCACGCTTGCGCGAGGA 3′(BamHI) | Cloning of ampG of PA01 | | PAampG-R | 5′GAAGCTTTCAGTGCTGCTCGGCGTTCTGGT3′(HindIII) | | | PAampG-I53S | 5′CCCAGCCAACTGGCGAAACCgctGGTATCCCGCGCCACGC3′ | Forward primer for I53S | | PAampG-I53T | 5′CCCAGCCAACTGGCGAAACCggtGGTATCCCGCGCCACGC3′ | Forward primer for I53T | | PAampG-53R | 5′GGTTTCGCCAGTTGGCTGGGGCTGGTGTACGCCTTCAAGT3′ | | | PAampG-W90R | 5′AGCACCTGCGAGAACACCAGccgGGAACGGCGCCGGCCGA3′ | Forward primer for W90R | | PAampG-W90S | 5′AGCACCTGCGAGAACACCAGcgaGGAACGGCGCCGGCCGA3′ | Forward primer for W90S | | PAampG-90R | 5′CTGGTGTTCTCGCAGGTGCTGATCGCCCTGGGACTGCTCG3′ | |
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Underlined are restriction endonuclease sites; nucleotide sequence corresponding to the mutated amino acids is shown in lowercase. |
