A Unique Combination of Mn²⁺ and Aluminum Adjuvant Acted the Synergistic Effect

Figure 1

Identification of IsdB3 proteins. (a) Confirmation of IsdB3 proteins by using SDS-PAGE. Lane 1: protein marker; lane 2 and 3: E. coli BL21 and E. coli BL21 with pET-32a (+) induced by IPTG as control, respectively; lane 4: E. coli BL21 with pET-32a (+)—Isdb3 induced by IPTG; and lane 5: the purification of IsdB3 proteins. (b) The IsdB3 proteins were detected with western blotting. Lane 1: the purification of IsdB3 proteins; lane 2: E. coli BL21 with pET-32a (+)—Isdb3; lane 3: E. coli BL21 with pET-32a (+); and lane 4: E. coli BL21.

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