Research Article
Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques
Figure 2
HLA-DR antibody for LC in vivo tracking. In vivo (a) representative images of Langerhans cells 2 h after in vivo injection of monoclonal antibodies HLA-DR-F647 and CD1a-AF488 (). Scale bars: 50 μm. (b) Flow cytometry analysis of fluorescently labeled immune cells isolated from skin biopsy 2 h after i.d. injection of monoclonal fluorescently labeled HLA-DR antibody (clone L243) or its isotype control. Skin cell suspensions were stained ex vivo with CD45 and HLA-DR (clone G46-6) antibodies. Transversal (c) section of skin biopsies and longitudinal (d) section of epidermis frozen 2 h after injection of fluorescent antibodies. Dotted lines split epidermal (left) and dermal (right) layers.
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