Isolated Perfused Rat Livers to Quantify the Pharmacokinetics and Concentrations of Gd-BOPTA

Figure 5

Canalicular transport across Mrp2. (a) Bile flow rates (µl/min/g) during the perfusion of Gd-DTPA, Gd-BOPTA, and MEB. (b) Plots between drug bile excretion rates (x-axis, nmol/min) and bile flow rates (y-axis, nl/min). (c) Concentration gradients between bile canaliculi and hepatocytes over time. (d) Plots between drug hepatocyte concentrations (x-axis, µM) and drug bile concentrations (y-axis, µM). Three groups of rat livers are perfused: (1) livers isolated from normal rats and perfused with 200 μM Gd-DTPA and 200 μM Gd-BOPTA (black circles, n = 5); (2) livers isolated from rats lacking Mrp2 and perfused with 200 μM Gd-DTPA and 200 μM Gd-BOPTA (green circles, n = 3); and (3) livers isolated from normal rats and perfused with 64 μM DTPA and 64 μM MEB (blue circles, n = 5). Adapted from [15] with permission.

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