Functionalization of Gadolinium Chelates Silica Nanoparticle through Silane Chemistry for Simultaneous MRI/⁶⁴Cu PET Imaging

Figure 2

Physicochemical characterization during the functionalization of GdNP. (a) DLS diagrams of SiGdNP (black), SiGdNP @ D-1 (DOTAGA) (blue), and SiGdNP @ N-1 (NODAGA) (red). (b) HPLC chromatograms of SiGdNP (before functionalization) (black) and SiGdNP @ D-1 (after functionalization with DOTAGA at 80°C) (red). (c) HPLC chromatograms of SiGdNP (before functionalization) (black) and SiGdNP @ N-1 (after functionalization with NODAGA at 80°C) (red). The concentration of samples analyzed by HPLC was at 5 mM in Gd. Eu titration curves λ_ex = 395 nm, λ_em = 594 nm of SiGdNP (d), SiGdNP @ D-1 (e), and SiGdNP @ N-1 (f).

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