InVivo Molecular Ultrasound Assessment of Glioblastoma Neovasculature with Endoglin-Targeted Microbubbles

<div>Preparation procedures of the microbubbles (MBs). Two types of MBs were prepared: (1) CD105-targeted MBs as MBs-biotin-avidin-biotin-CD105-biotin and (2) control nontargeted MBs as MBs-biotin-avidin-biotin-IgG-biotin. Biotinylated rat antimouse CD105 monoclonal antibodies (eBioscience) and biotinylated rat immunoglobulin G antibodies (IgG) were used as 1st antibodies for the CD105-targeted MBs and the control nontargeted MBs, respectively. The 2nd antibodies, which were fluorescein-conjugated antibiotin antibodies (Jackson ImmunoResearch), were used to confirm the affinity of the 1st antibodies on the shell of MBs through the specific biotin-avidin conjugation.</div>

Contrast Media & Molecular Imaging

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Figure 2: InVivo Molecular Ultrasound Assessment of Glioblastoma Neovasculature with Endoglin-Targeted Microbubbles