Imaging Characteristics of USPIO Nanoparticles (<5 nm) as MR Contrast Agent <i>In Vitro</i> and in the Liver of Rats

<div>The influence of 5 nm USPIO nanoparticles on the cell viability and uptake of BRL-3A cells determined by MTT and Prussian blue staining assay. (a) Statistical analysis of MTT results revealed that only 24 hours incubation of 200 <i>μ</i>g·Fe/mL and 400 <i>μ</i>g·Fe/mL USPIO nanoparticles significantly weakened the cell viability as compared with the control (<span class="nowrap"><svg height="13.532pt" id="M3" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.532" width="50.6052pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 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-3Z"></path></g><g transform="matrix(.013,0,0,-0.013,34.265,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.505,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.469,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.709,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g 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For each group in MTT assay, 5 wells per plate were treated identically. (b, c) Cellular uptake of 5 nm USPIO nanoparticles determined by iron Prussian blue staining assay. Rat normal hepatic cells BRL-3A were treated by USPIO nanoparticles at 20, 50, and 100 <i>μ</i>g·Fe/mL for 6 and 12 hours, as compared with the control group treated by culture media.</div>

Contrast Media & Molecular Imaging

fig3

Figure 3

Figure 3: Imaging Characteristics of USPIO Nanoparticles (<5 nm) as MR Contrast Agent <i>In Vitro</i> and in the Liver of Rats 

Contrast Media & Molecular Imaging

Imaging Characteristics of USPIO Nanoparticles (<5 nm) as MR Contrast Agent In Vitro and in the Liver of Rats

Figure 3