Paired-Agent Fluorescence Molecular Imaging of Sentinel Lymph Nodes Using Indocyanine Green as a Control Agent for Antibody-Based Targeted Agents

Figure 4

Kinetics of the antibody-based targeted agent (IRDye-700DX-cetuximab) and ICG signal in rats metastatic popliteal lymph nodes over time after intradermal footpad injection. The potential of using ICG as the control agent to detect metastases in n = 4 rats tumor-draining lymph nodes are presented in (a)–(d). The binding potential as a function of time for various lymph nodes after injection is presented in (e), including the binding potential of a control node. The presence of cancer in all metastatic lymph nodes was validated based on the presence of green fluorescent protein (GFP) fluorescence signal in microscopy since the cells were transfected with the gene for GFP, for example, which is presented in (f).

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