Paired-Agent Fluorescence Molecular Imaging of Sentinel Lymph Nodes Using Indocyanine Green as a Control Agent for Antibody-Based Targeted Agents

Figure 7

Influence of the mixing order and preloading time of ICG and albumin on ICG fluorescence. Fluorescence from ICG varied when mixing in different orders of ICG, albumin, and antibody-based agent (AI) IRDye-700DX-IgG as presented in (a). ICG preloaded with albumin prior to mixing with the antibody-based agent allowed ICG to reach its highest intensity without quenching its fluorescence. The fluorescence profile of ICG in albumin as a function of time is shown in (b). Fluorescence intensity of ICG was maximum at 15 min, suggesting that ICG has completely bound to albumin at this point.

(a)

(b)