^99mTc-Labeled LyP-1 for SPECT Imaging of Triple Negative Breast Cancer

Figure 3

Cellular uptake of LyP-1-FITC and ARAL-FITC by 4T1 cells was examined by flow cytometry. Cells were treated with the drug-free medium (a), ARAL-FITC (b), or LyP-1-FITC (c) at a concentration of 10 μM for 2 h, and the statistical analysis results are shown in (d). Cells treated with 10 μM ARAL-FITC (e) and LyP-1-FITC (f) were analyzed after incubation for 3 h, and the difference was compared in (g). The relative fluorescence signals of cells treated with ARAL-FITC (h) or LyP-1-FITC (i) were compared for 2-3 h incubation, respectively.

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(d)

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