Research Article

Coarse-Grained Simulation of Myosin-V Movement

Table 2

Binding state coexistence in the two myosin “legs.” The binding state of each myosin leg can be either free, in loose or tight binding or in the latter state after the powerstroke has swung. Combinations of these four states were counted for both leg-1 and leg-2 over ten circuits of the track. Counts were tabulated for the default model (good leg) and a more flexible myosin (weak leg). As no significant difference was seen between symmetric counts (leg-1/2 and leg-2/1), these were combined and the (weak-good) difference taken.

Leg-1 stateLeg-2 state
FreeLooseTightSwung

Good leg

Free04542799644
Loose71210930995
Tight215025810333
Swung759154490

Weak leg

Free07212262977
loose801199435178
Tight319024710639
Swung1505163570

Combined (weak good)

Free116649491403
Loose1522567249
Tight545268282
Swung248234196

Difference (weak-good)

Free0
Loose35690
Tight503115−3
Swung107992140