Table 1: Results of automated segmentation performance on 21 test images.

StainID/magnificationManual quantification (cells)Automated quantification (cells)Accuracy (%)Precision (%)Sensitivity (%)Specificity (%)

SMActinDay 5–40x10110092.969190.0194.67
Day 5–60x596091.8288.3389.8393
Day 7–40x16618793.5585.5696.3892.19
Day 7–60x879493.9689.2495.4093.10
Day 14–40x858892.7589.7792.9492.62

H&EDay 5–40x8686100100100100
Day 7–40x14113899.0510097.87100
Day 7–60x667195.1090.1496.9694.06
Day 14–40x10510898.6997.2210097.6
Day 14–60x423895.2810088.095100

CD-31Day 5–40x767799.3898.7010098.85
Day 5–60x524997.4310094.23100
Day 7–40x14018894.3277.7797.2293.66
Day 7–60x615997.6110095.08100
Day 14–40x9810497.9794.2310097.97
Day 14–60x6767100100100100

CD-68Day 5–40x999691.3676.1996.9689.39
Day 5–60x686695.1384.6197.0594.54
Day 14–40x14313898.5510096.50100
Day 14–60x697099.4998.5710099.23

Total18111884
Average95.5591.2796.4595.07