Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences

<div>The process of detecting a fusion candidate vesicle. (a) A patch of image with a fusion candidate vesicle in. (b) The absolute differentiation of consecutive images results in a noisy difference image, from which the vesicle is hard to detect. (c) The forward moving average differentiation can achieve a high SNR difference image. (d) A vesicle spot mask derived from (c) with the MAD threshold, which can be used to calculate the intensity-weighted centroid (a red cross).</div>

Computational and Mathematical Methods in Medicine

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Figure 2: Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences 