| (1) Biochem Biophys Res Commun. |
| 2006 Sep 22;348(2):514-23. |
| Epub 2006 Jul 28. |
| Aurelin, a novel antimicrobial peptide |
| from jellyfish Aurelia aurita with |
| structural features of defensins |
| and channel-blocking toxins. |
| Ovchinnikova TV, Balandin SV, Aleshina |
| GM, Tagaev AA, Leonova YF, Krasnodembsky |
| ED, Men’shenin AV, Kokryakov VN. |
| Shemyakin and Ovchinnikov Institute of |
| Bioorganic Chemistry, Russian Academy of |
| Sciences, Miklukho-Maklaya str. 16/10, |
| 117997 Moscow, Russia. [email protected] |
| A novel 40-residue antimicrobial peptide, |
| aurelin, exhibiting activity against |
| Gram-positive and Gram-negative bacteria, |
| was purified from the mesoglea of a |
| scyphoid jellyfish Aurelia aurita by |
| preparative gel electrophoresis and RP-HPLC. |
| Molecular mass (4296.95 Da) and complete |
| amino acid sequence of aurelin |
| (AACSDRAHGHICESFKSFCKDSGRNGVKLRANCKKTCGLC) |
| were determined. Aurelin has six |
| cysteines forming three disulfide bonds. |
| The total RNA was isolated from the |
| jellyfish mesoglea, RT-PCR and cloning |
| were performed, and cDNA was sequenced. |
| A 84-residue preproaurelin contains a |
| putative signal peptide (22 amino acids) and |
| a propiece of the same size (22 amino acids). |
| Aurelin has no structural homology with any |
| previously identified antimicrobial peptides |
| but reveals partial similarity both with |
| defensins and K+ channel-blocking toxins |
| of sea anemones and |
| belongs to ShKT domain family. |
| PMID: 16890198 [PubMed -indexed for MEDLINE] |
