Use of Poly (-Lysine) Dendrons: A Strategy Targeting Bacterial Quorum Sensing and Biofilm Formation
Figure 3
Effects of hyperbranched poly (-lysine) dendrons of various lysine generation numbers, in the presence or absence of ciprofloxacin, on biofilm formation of Pseudomonas aeruginosa (NCTC 10662). Biofilms of PA10662 were cultured on stainless steel discs for 30 minutes or 48 h. Discs were then treated for 1 h with poly (-lysine) dendrons (RGnK) at an equimolar concentration of amine groups; (a) RG1K, 1600 μM; (b), RG2K, 800 μM; (c), RG3K, 400 μM; CIP, Ciprofloxacin (0.5 μg/mL); RGnK ≫ CIP, administration of RGnK followed by ciprofloxacin (0.5 μg/mL); RGnK + CIP, coadministration of RGnK and ciprofloxacin (0.5 μg/mL). Percentage of viable cells recovered from each disc was then determined to assess the ability of each treatment to disperse established biofilms. The data represent mean values of experiments performed in duplicates. Error bars are means ± SD. ; ; (versus ciprofloxacin), Student’s -test.
