Use of Poly (-Lysine) Dendrons: A Strategy Targeting Bacterial Quorum Sensing and Biofilm Formation
Figure 4
Quorum sensing inhibitory activity of poly (-lysine) dendrons against pyocyanin production. Cultures of Pseudomonas aeruginosa (PAO1) were grown in the presence or absence of either generation 3 poly (-lysine) dendrons (RG3K, 400 μM) and/or ciprofloxacin (CIP, 0.5 μg/mL). The percentage production of pyocyanin was determined 20 h after treatment. (a) Effect of poly (-lysine) dendrons alone and in combination with ciprofloxacin on pyocyanin production in PAO1. Cultures were exposed to (i) Nontreated control, (ii) RG3K (400 μM), (iii) Ciprofloxacin (0.5 μg/mL), and a combination of RG3K (400 μM) and ciprofloxacin (0.5 μg/mL). (b) Quantitative analysis of pyocyanin production in PAO1. The data represent mean values of experiments performed in triplicates. Error bars are means ± SD. , Student’s -test.
