Differential Effect of Hypoxia in Human and Mouse Vascular Smooth Muscle Cell Migration through LRP1-pPyk2-MMP-9 Axis

Figure 1

Time-course of hypoxia induced Pyk2 and ERK1,2 phosphorylation in hVSMC and mVSMC. hVSMC and mVSMC were exposed to either normoxia or hypoxia for 4, 8, 16, and 32 hours. ((a), (e)) Representative Western blot analysis showing LRP1, pPyk2, total Pyk2, pERK1,2, and total ERK1,2 bands. β-tubulin was detected as loading control. Bar graphs show quantification of LRP1 ((b), (f)), pPyk2/total Pyk2 ratio ((c), (g)), and pERK1,2/total ERK1,2 ((d), (h)) in normoxic (gray bars) and hypoxic (black bars) conditions. Results are expressed as mean ± SEM of two independent experiments performed in triplicate. N, normoxia; H, hypoxia and versus normoxic cells.