Differential Effect of Hypoxia in Human and Mouse Vascular Smooth Muscle Cell Migration through LRP1-pPyk2-MMP-9 Axis

Figure 3

Time-course of the hypoxic modulation of MMP-9/MMP-2 expression and activation in hVSMC and mVSMC. hVSMC and mVSMC were exposed to either normoxia or hypoxia for increasing times and harvested to assess MMP-9 and MMP-2 expression using quantitative RT-PCR ((a) and (d), resp.). Endogenous gene reference was TBP and ARBP for human and mouse, respectively. Representative zymographic analysis ((b) and (e), resp.) detecting MMP-9 and MMP-2 in conditioned media from normoxic and hypoxic cells and bar graphs showing the quantification of bands ((c) and (f), resp.). Results from two experiments performed in duplicate were expressed as mean ± SEM. versus normoxic cells.