Differential Effect of Hypoxia in Human and Mouse Vascular Smooth Muscle Cell Migration through LRP1-pPyk2-MMP-9 Axis

Figure 4

Effect of PP2 and U0126 on hypoxia-induced VSMC migration. Quiescent hVSMC or mVSMC were untreated or treated with PP2 (50 μM) or U0126 (10 μM) for different times under normoxic or hypoxic conditions. Phase-contrast microscopy images showing the migratory capacity of control, PP2-treated and U0126-treated hVSMC ((a) and (b)), and mVSMC ((c) and (d)) in normoxia and hypoxia and bar graphs show quantification of cell migration rate over time. Data from two independent experiments performed in triplicate are expressed as mean ± SEM. versus normoxic cells, versus control.

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