The Decrease of n-3 Fatty Acid Energy Percentage in an Equicaloric Diet Fed to B6C3Fe Mice for Three Generations Elicits Obesity
Table 3
The effect of long-lasting low n-3 fatty acid diet on mouse liver fatty acid concentrations in total lipid and total phospholipid extracts. The results are expressed as mean SD of 5 measurements per group.
Fatty acids in total lipid and total phospholipid liver extracts (g / mg tissue)
Total Lipids
Total Phospholipids
Standard n-3
Low n-3 FA
Standard n-3
Low n-3 FA
sum saturates
15.2 ± 3.2
18.7 ± 8.1
2.72 ± 0.7
1.98 ± 0.6
sum monosats.
12.3 ± 7.3
21.6 ± 11.6
1.24 ± 0.22
0.93 ± 0.31
18:2n-6
5.55 ± 1.14
0.23 ± 0.22
1.34 ± 0.49
0.97 ± 0.36
18:3n-6
0.088 ± 0.02
0.39 ± 0.34
0.020 ± 0.008
0.017 ± 0.007
20:2n-6
0.17 ± 0.046
0.66 ± 0.61
0.041± 0.018
0.030 ± 0.015
20:3n-6
0.70 ± 0.11
1.16 ± 0.70
0.17 ± 0.059
0.13 ± 0.063
20:4n-6
5.54 ± 0.38
6.64 ± 1.0
1.64 ± 0.55
1.36 ± 0.61
22:2n-6
0.13 ± 0.008
0.077 ± 0.065
0.00 ± 0.00π
0.00 ± 0.00π
22:4n-6
0.18 ± 0.05
0.68 ± 0.48
0.033 ± 0.016
0.037 ± 0.021
18:3n-3
0.087 ± 0.037
0.068 ± 0.061
0.01 ± 0.004
0.00 ± 0.00*
20:3n-3
0.012 ± 0.007
0.011 ± 0.013
0.002 ± 0.001
0.001 ± 0.001
20:5n-3
0.097 ± 0.013
0.027 ± 0.015*
0.022 ± 0.0014
0.00 ± 0.00*
22:5n-3
0.17 ± 0.04
0.055 ± 0.025*
0.037 ± 0.017
0.01 ± 0.004*
22:6n-3
2.37 ± 0.27
0.78 ± 0.093*
0.73 ± 0.34
0.24 ± 0.08*
sum n-6ν
6.39 ± 0.47
9.22 ± 2.75
1.89 ± 0.63
1.55 ± 0.68
sum n-3ν
2.65 ± 0.26
0.88 ± 0.14*
0.80 ± 0.37
0.25 ± 0.085*
% n-6 HUFA
70.7 ± 2.55
91.1 ± 1.28*
70.3 ± 8.6
84.7 ± 6.5*
um n-6 and sum n-3 include the critically bioactive 20 and 22 carbon fatty acids only. The n-6 % HUFA is calculated as: (sum n-6)/[(sum n-6)/(sum n-3)]. 100. oncentrations are lower than 0.01. The statistical significance was calculated using an independent samples T-test as described in methods (Sig. 2-tailed: ).
