The Decrease of n-3 Fatty Acid Energy Percentage in an Equicaloric Diet Fed to B6C3Fe Mice for Three Generations Elicits Obesity

Table 4

The effect of low n-3 fatty acid diet on stearyl-coenzyme A desaturase 1 (Scd1) and stearyl-coenzyme A desaturase 2 (Scd2) gene regulation in mouse liver. Rt-PCR was performed on total RNA extracts from mouse livers (SABiosciences, Frederick, MD). Ct values corrected against house keeping gene, beta actin, are expressed as average of 4 measurements per group.

Gene	▵Ct Standard n-3	▵Ct Low n-3	Fold Change
Scd1	−2.55	−5.24	6.46
Scd2	10.98	10.83	1.1