The Calcium-Dependent Interaction of S100B with Its Protein Targets
Figure 7
S100A1-488 binding curves for glycogen phosphorylase a. Membranes containing varying concentrations of glycogen phosphorylase were incubated in S100A1-488 in the presence () or absence (■) of Ca2+. A standard curve of fluorescence intensity per mg of S100-488 was used to determine the experimental amount of labeled S100 per dot of target protein (n 17).