Chromatography Research International

Research Article

A Validated High-Performance Liquid Chromatography-Tandem Mass Spectrometric (Lc-Ms/Ms) Method for Simultaneous Determination of R(+)-Ketorolac and S(−)-Ketorolac in Human Plasma and Its Application to a Bioequivalence Study

Table 4

Intra- and interday precision and accuracy for determination of ketorolac enantiomers in human plasma by LC-MS/MS.
Nominal concentration (ng/ml)Observed ( m e a n ± S . D .) (ng/ml)%CV%AccuracyNumber of observations ( 𝑛 )
Intra-day
R(+)-ketorolac
9.37 (LOQQC) 9 . 4 3 ± 0 . 3 3 3.5100.712
24.57 (LQC) 2 4 . 0 1 ± 0 . 7 6 3.297.712
511.90 (MQC) 5 2 5 . 3 7 ± 1 5 . 5 2 3.0102.612
984.42 (HQC) 9 7 2 . 5 7 ± 2 3 . 1 5 2.498.812
S( − )-ketorolac
6.09 (LOQQC) 5 . 9 5 ± 0 . 2 4 4.197.712
15.95 (LQC) 1 4 . 2 4 ± 0 . 5 6 3.989.312
332.35 (MQC) 3 1 3 . 9 3 ± 7 . 5 7 2.494.512
639.14 (HQC) 5 7 2 . 9 3 ± 9 . 9 1 1.789.612
Inter-day
R(+)-ketorolac
9.37 (LOQQC) 9 . 3 3 ± 0 . 5 9 6.399.518
24.57 (LQC) 2 4 . 1 7 ± 1 . 0 0 4.198.418
511.90 (MQC) 5 2 5 . 9 5 ± 2 7 . 5 6 5.296.918
984.42 (HQC) 9 7 3 . 8 5 ± 4 6 . 1 8 4.798.918
S( − )-ketorolac
6.09 (LOQQC) 5 . 9 1 ± 0 . 2 8 4.797.018
15.95 (LQC) 1 4 . 5 9 ± 0 . 9 7 6.791.518
332.35 (MQC) 3 2 1 . 9 8 ± 1 6 . 8 7 5.296.918
639.14 (HQC) 5 8 8 . 6 5 ± 3 2 . 1 3 5.592.118