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Chromatography Research International
Volume 2011 (2011), Article ID 232505, 3 pages
Research Article

Validated RP-HPLC Method for the Determination of Buspirone in Pharmaceutical Formulations

1Department of Chemistry, Krishna University, Machilipatnam 521001, A.P, India
2Department of Chemistry, GITAM University, Visakhapatnam 530045, A.P, India

Received 10 June 2011; Revised 8 July 2011; Accepted 31 July 2011

Academic Editor: Irene Panderi

Copyright © 2011 M. V. Basaveswara Rao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the rapid assay of the Buspirone in tablet dosage form. Isocratic elution at a flow rate of 1.0 mL/min. was employed on a symmetry C18 ( 2 5 0 × 4 . 6  mm, 5 μm in particle size) at ambient temperature. The mobile phase consisted of water : acetonitrile : methanol 45 : 35 : 20(V/V). The UV detection wavelength was 210 nm, and 20 μL sample was injected. The retention time for the Buspirone was 7.057 min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method can be successfully applied for the routine analysis of Buspirone in tablet dosage form.