Research Article

A Simple Densitometric Method for the Quantification of Inhibitory Neurotransmitter Gamma-Aminobutyric Acid (GABA) in Rat Brain Tissue

Table 1

Method validation parameters for the quantification of GABA by HPTLC method.

ParametersResults

Instrumental precision (% CV)0.5891
Reproducibility (% CV)0.4141
Variability (% CV)
 (i) Inter-day0.9453
 (ii) Intra-day1.3236
Limit of detection (ng)1
Limit of quantification (ng)10
SpecificityPrecise
Linearity (correlation coefficient)0.99940
Range (ng spot−1)5–80