Research Article
Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms
Table 6
Robustness of the method.
| Parameter | | Amount of vinpocetine added (μgmL−1) | Amount of vinpocetine detected (Mean ± SD)* | %RSD |
| Change in mobile phase composition | Buffer : ACN = 38 : 62 | 200 | 200.33 ± 0.29 | 0.14 | Buffer : ACN = 40 : 60 | 200 | 200.47 ± 0.29 | 0.14 | Buffer : ACN = 42 : 58 | 200 | 200.56 ± 0.55 | 0.27 | Change in column temperature | 25°C | 200 | 200.43 ± 0.71 | 0.35 | 30°C | 200 | 200.36 ± 0.50 | 0.23 | 35°C | 200 | 199.88 ± 0.56 | 0.28 | Change in flow rate | 0.9 mLmin−1 | 200 | 200.08 ± 0.65 | 0.33 | 1.0 mLmin−1 | 200 | 200.41 ± 0.31 | 0.15 | 1.1 mLmin−1 | 200 | 200.46 ± 0.61 | 0.31 |
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*Mean ± Standard Deviation of six replicates.
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