Development and Validation of Selective High-Performance Liquid Chromatographic Method Using Photodiode Array Detection for Estimation of Aconitine in Polyherbal Ayurvedic Taila Preparations

Table 1

Summary of validation parameters and system suitability parameters.

Parameters	Observations  ±  % RSD (𝑛=06)
Retention time (min)	42.54±0.33
Tailing factor	1.1±0.1
Resolution	1.7±0.23
Theoretical plates	3213±0.42
Linearity range (μg/mL)	15–90
Correlation coefficient (𝑟^2)	0.9989
Regression equation	𝑦=71.5𝑥−24.5
Limit of detection (μg/mL)	0.03
Limit of quantification (μg/mL)	0.1
Repeatability (% RSD, 𝑛=6)	0.55–1.68