HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

Table 1

Calibration curve data for RT- F2 compound of HPTLC method.

Track	𝑅𝑓	Concentration of RT-F2	Height of peak	Calculated RT-F2	Area of peak	Calculated RT-F2
1	0.57	1.250 μg	51.72		641.96
2	0.56	2.500 μg	48.37		1005.73
3	0.56	3.750 μg	71.26		1446.38
4	0.56	Unknown*	73.75	3.758 μg	1558.45	4.006 μg
5	0.57	Unknown**	158.38	>6.875 μg	3246.91	>6.875 μg
6	0.57	5.000 μg	86.79		1945.75
7	0.58	6.250 μg	110.10		2313.74

*Petroleum ether extract, **Ethyl acetate extract
Regression equation (Height) 𝑌=27.094+12.415𝑋, 𝑟=0.9574
Regression equation (Area) 𝑌=185.641+342.686𝑋, 𝑟=0.9986.