HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

Table 2

Quantification of RT-F2 compound.

Stationary phase	Precoated Silica Gel 60 GF254
Mobile phase	Chloroform : toluene : ethyl acetate (6 : 3 : 1)
Calibration range of F2	2.5–12.5 μg/spot
Detection	Anisaldehyde sulphuric acid reagent heated at 110°C for 5 min and detected at 600 nm.
Regression equation	𝑌=185.6+342.686𝑋 (area wise)
𝑅 value	0.99862 (area wise)