Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts

Table 2

Accuracy results of MLC method: analysis of sesquiterpenic acid mixtures and standard addition technique.

Compound	Added (μg·mL^−1)	As found*(μg·mL^−1)	% Recovery	Δ	Added, μg·mL^−1	As found*, μg·mL^−1		Recovery, %
						with addition	without addition
Hydroxyvalerenic acid	1.90	1.88	99.0	−1.0	0	—	2.10
	9.30	9.25	99.5	−0.5	1.90	4.20	2.20	104.8
	27.9	27.8	99.6	−0.4	5.60	7.74	2.14	102.1
					9.3	11.4	2.13	101.7
	Mean, %R.S.D.		99.4, 0.8				Mean, %	102.9
Acetoxyvalerenic acid	4.20	4.19	99.8	−0.2	0	—	33.4
	21.0	20.85	99.3	−0.7	12.6	46.3	33.7	102.1
	63.0	63.1	100.1	+0.1	21.0	54.2	33.2	99.4
					29.4	63.5	34.1	102.1
	Mean, %R.S.D.		99.7, 1.1				Mean, %	101.2
Valerenic acid	6.10	6.07	99.5	−0.5	0	—	50.7
	30.4	30.5	100.3	+0.3	18.2	69.4	51.2	101.0
	91.2	91.4	100.2	+0.2	30.4	80.7	50.3	99.2
					42.6	93.6	51.0	100.6
	Mean, %R.S.D.		100.0, 1.1				Mean, %	100.3

*Mean value of the four determinations.