CZE/PAD and HPLC-UV/PAD Profile of Flavonoids from Maytenus aquifolium and Maytenus ilicifolia “espinheira santa” Leaves Extracts

Table 1

Effect of pH variation on the values of migration time (𝑡_M) and electrophoretic mobility (𝜇_ef) of flavonoid standards: (1) rutin, (2) kaempferol, and (3) quercetin. Buffer: tetraborate 30 mmol/L/phosphate 5 mmol/L. For detailed CZE conditions, see experimental section.

pH	𝑡M(1) (min)	𝑡M(2) (min)	𝑡M(3) (min)	𝜇ef(1)×10^−4 (cm^2V⋅s)	𝜇ef(2)×10^−4 (cm^2V⋅s)	𝜇ef(3) ×110^−4 (cm^2V⋅s)
8.0	9.625	8.983	12.399	1.671	1.433	2.372
8.5	9.512	9.597	12.613	1.651	1.679	2.430
9.0	9.347	10.587	12.869	1.850	2.228	2.733
9.3	10.040	12.537	14.576	1.837	2.435	2.772
9.5	10.248	13.742	15.375	1.880	2.628	2.861
10.0	11.475	18.693	19.741	1.940	2.954	3.040

𝜇_ef=(𝐿_t×𝐿_ef)/(𝑡_m×𝑉)−(𝐿_t×𝐿_ef)/(𝑡_nm×𝑉), where 𝐿_t is the total lenght of the capillary, 𝐿_ef is the effective lenght of the capillary,𝑡_m is the migration time of the analyte, 𝑡_nm is the migration time of the neutral marker (methanol), and 𝑉 is the applied voltage.