Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation
Figure 1
Resolution of two adjacent peaks of DKPs, dipeptides, and amino acids depending on MeOH concentration (a–c) or flow rate (d); substances with (a) 10% MeOH, (b) 20% MeOH, and (c) 30% MeOH chosen as best separation conditions; (d) cyclo(L-Asp-L-Asp) and L-Asp-L-Asp at flow rate from 0.3 to 0.7 mL min−1 at 15 and 20°C column temperature and 10% MeOH.
