Research Article

Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form

Table 3

Optimized chromatographic conditions.

ParameterOptimized condition

ChromatographShimadzu HPLC with UV detector
ColumnPhenomenex C18 column (250 × 4.6 mm, 5  )
Mobile phase1% o-phosphoric acid in water, pH adjusted to 3.0 : acetonitrile : methanol (78 : 10 : 12)
Flow rate1 mL/min
DetectionUV at 254 nm
Injection volume20  L
Column temperature23°C