Research Article

Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form

Table 1

Validation parameters for CPM, IBU, and PHE.

ParametersCPMIBUPHE

Linearity (range) (µg/mL)0.5–2.525–1251.25–6.25
Retention time (min)4.213.62.7
Detection limit (µg/mL)0.03210.11980.0679
Quantitation limit (µg/mL)0.5251.25
Accuracy (%) 99.44–101.61 99.39–101.79 99.66–101.83
Precision (RSD%)a
 Intraday precision ( ) 0.44–1.280.10–0.23 0.38–0.56
 Interday precision ( )0.98–1.46 0.66–1.33 0.66–1.53
 Instrument precision (RSD%)a ( )1.380.570.12

RSD is relative standard deviation and “n” is number of determinations.