A New Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Pyridoxine Hydrochloride and Meclizine Hydrochloride in Pharmaceutical Solid Dosage Forms

Table 8

Robustness of the method.

Parameters	Actual Variance	Amount added (mg mL^−1)		% Recovery (Mean ±  %RSD)*
		PYH	MEH	PYH	MEH
Flow Rate	0.48 mL min^−1	0.5	0.25	100.55±0.33	100.04±0.04
	0.52 mL min^−1	0.5	0.25	100.12±0.02	100.18±0.07
Organic (%) in mobile phase	68.7	0.5	0.25	99.95±0.22	100.15±0.04
	71.3	0.5	0.25	99.75±0.51	100.18±0.07
Detector wavelength	252 nm	0.5	0.25	100.00±0.15	99.81±0.19
	256 nm	0.5	0.25	100.00±0.11	99.53±0.37
Column Temperature	39°C	0.5	0.25	100.17±0.20	100.08±0.11
	35°C	0.5	0.25	100.41±0.46	100.07±0.05

∗Mean of three replicates.