Ewing Sarcoma/Primitive Neuroectodermal Tumor of the Kidney: Two Unusual Presentations of a Rare Tumor

Table 1

Specifications of the antibodies used in the study.

Antibody	Clone	Company	Dilution	Retrieval
NSE	O10	Immunotech (France)	PD	CC1 mild 30”
CD56	7	Vector (US)	1/10	CC1 mild 30”
CKAE1/3	PGM-1	DAKO (Denmark)	1/100	CC1 mild 30”
Cam 5.2	KP-1	VENTANA (USA)	PD	CC1 mild 30”
PGP9.5	10D6	VECTOR (UK)	1/250	CC1 mild 30”
Bcl2	Polyclonal	NOVACASTRA (UK),	1/500	None
CD99	12D6	VECTOR (UK)	1/100	CC1 mild 30”
Synaptophysin	Polyclonal	GENETEX, Inc. (USA)	1/25	Protease digestion 4”
EMA	55K-2	DAKO (Denmark)	1/500	CC1 mild 30”
Ki67	MIB-1	DAKO (Denmark)	1/25	CC1 mild 30”
Inhibin	RI’	DAKO (Denmark)	1 : 50	CC1 standard 60”
Chromogranin	LK2H10	VENTAN (USA)	PD	None
Vimentin	V9	VENTANA (USA)	PD	CC1 standard 60”
WT1	6F-H2	DAKO (Denmark)	1 : 25	CC1 standard 60”
S100	Polyclonal	DAKO (Denmark)	1 : 500	CC1 short 8”
CK7		DAKO (Denmark)	1 : 200	Protease 24”
ER	SP1	VENTANA (USA)	PD	CC1 standard 60”
Pan-CK		ABCAM (USA)	1 : 100	CC1 mild 30”
CAM5.2	CAM5.2	Beckton-Dickison (USA)	1 : 10	Protease 18”
Myogenin		VENTANA (USA)	PD	CC1 standard 60”
TTF1	B72.3	BIOGENEX (USA)	1 : 50	CC1 mild 30”
CK20	K5208	DAKO (Denmark)	1 : 50	CC1 mild 30”
BHCG		VENTANA	PD	CC1 mild 30”
AFP	Polyclonal	DAKO (Denmark)	1 : 2000	CC1 standard 60”
CD30	BER-H2	VENTANA (USA)	PD	CC1 standard 60”
CD20	L26	VENTANA (USA)	PD	CC1 mild 30”
CD3	Polyclonal	DAKO (Denmark)	1 : 100	CC1 mild 30”
CD43	L60	VENTANA (USA)	PD	CC1 short 8”
LCA	RP2/18	VENTANA (USA)	PD	CC1 mild 30”
CA9	Polyclonal	NOVUS BIOLOGICALS (USA)	1 : 1000	CC1 mild 30”
CD10		CELL MARQUE (USA)	PD	CC1 standard 60”
AR	AR441	DAKO (Denmark)	1 : 100	CC1 standard 60”
Desmin	DE-R-11	VENTANA (USA)	PD	CC1 mild 30”
SMA	IA4	VENTANA (USA)	PD	None
HMB45	CMA710	CELL MARQUE (USA)	PD	CC1 mild 30”

CC1: Cell Conditioning 1 is antigen retrieval from Ventana Benchmark similar to high pH EDTA buffer. PD: prediluted, CA9: carbonic anhydrase 9, SMA: smooth muscle actin, AR: Androgen receptor, AFP: alpha-fetoprotein, TTF1: thyroid transcription factor-1, ER: estrogen receptor, and NSE: neuron-specific enolase.