Clonality Assessment in a Case of Multifocal Adamantinoma and a Review of the Literature

Figure 8

Analysis of electrophoretic band patterns: X chromosome inactivation pattern according to a methylation-sensitive PCR of the FMR1 gene region. A 100% skewing of the tibia tumour (TU I) as well as the fibula tumour (TU II) could be detected (presence of a single cell line consisting of the methylated (M) allele 2 and the unmethylated (UM) allele 1). The analysis of the distal lesion of the tibia (TU III) as well as the peripheral blood (PB) showed a random X inactivation (four distinct bands of equal intensity that represent the two methylated and unmethylated alleles).