Case Report

An Extremely Rare, Remote Intracerebral Metastasis of Oral Cavity Cancer: A Case Report

Figure 3

Histology and immunohistochemistry of OSCC, 5 μm thick serial sections of both primary tumor and cerebral metastasis were stained with H&E. Immunochemistry was performed using an indirect peroxidase system with nonbiotinylated polymer secondary antibodies following the instructions of the manufacturer (MEDAC). Diaminobenzidine (Sigma, brown) is used as a chromogen. Magnification: original 20. (a) Primary intermediately differentiated squamous cell carcinoma of the oral cavity with recognizable squamous cell differentiation. (b) Cerebral metastasis of poorly differentiated squamous cell carcinoma containing few horn pearls (arrow heads) and central necrosis. (c) Cerebral metastasis of OSCC, immunocytochemistry for CK 5/6, a cytokeratin marker indicative for squamous cell carcinoma with completely positive brown reaction product on the plasma membrane of nearly all tumor cells. Adjacent brain tissue shows gliosis but remains negative for CK-5/6 (light blue). (d) Cerebral metastasis of OSCC, immunohistochemistry for EGFR shows strong overexpression with complete staining of the cell membranes in all vital tumor cells. Note the negative results in remaining brain parenchyma (light blue).
257046.fig.003a
(a)
257046.fig.003b
(b)
257046.fig.003c
(c)
257046.fig.003d
(d)