Atypical Central Neurocytoma with Recurrent Spinal Dissemination over a Period of 20 Years: A Case Report and Review of the Literature
Figure 2
Tumor histomorphology at different time points. (a) In 2007, excised mostly monomorphic highly cellular tumor with regular rounded nuclei and only few mitoses. The blood vessels are regularly arranged resembling a loose meshwork without pathological endothelial proliferation or glycogen storage. (Periodic acid Schiff (PAS), Magnification original 20.) (b) Immunostaining for the proliferation marker K67 (MIB-1) showing only few proliferating cells (indirect peroxidise technique (LSABII, DAKO, diaminobenzidine (DAB) as a chromogen, brown, Magnification orig. 20, counterstaining with Hematoxylin)). (c) Immunostaining for -III-Tubulin (Chemicon), a marker of immature neural cells with strong positivity of all tumor cells. (LSABII, DAKO, (DAB), counterstaining with Hematoxylin Magnification orig. 20.) (d) Immunostaining for the neuronal marker synaptophysin (Dako) with strong positive staining of all tumor cells. (LSABII, DAKO, (DAB), counterstaining with Hematoxylin Magnification orig. 20.) (e) Immunostaining for chromogranin (Dako), a marker of highly differentiated neuronal cells and ganglion cells with mostly negative staining results. (LSABII, DAKO, (DAB), counterstains with Hematoxylin Magnification orig. 20.) (f) Immunostaining for S100 (Dako) showing a mostly negative staining result with single positive cells of glial morphology at irregular intervals (LSABII, DAKO, (DAB), counterstaining with Hematoxylin Magnification orig. 20.) (g) Tumor biopsy of 2006 with similar morphology of lower cellularity. Noteworthy are the occasional cell-free areas. (H&E, Magnification original 10.) (h) Tumor biopsy of 1992 showing similarly organized tumor cells with rounded nuclei and even less mitoses within an extensive hemorrhage. (H&E, Magnification original 20.)
