Case Report
A Histopathological and Immunohistochemical Analysis of Ameloblastic Fibrodentinoma
Table 1
The antibodies used to detect the expression of the different proteins in the epithelial and mesenchymal cells of the AFD samples.
| Antibody | Source/clone | Dilution | Antigen retrieval | Epithelial cells | Mesenchymal cells |
| Amelogenin | Santa Cruz/SC-33109 | 1 : 100 | Mw | + | Neg | CK19 | Genetex/polyclonal | 1 : 100 | Mw | +++ | Neg | Calretinin | DAKO/DAK-CALERET 1 | 1 : 100 | Mw | + | Neg | E-cadherin | DAKO/NCH-38 | 1 : 100 | Mw | ++ | Neg | Syndecan-1 | DAKO/MI15 | 1 : 100 | Mw | +++ | ++ | Histone H3 | GeneTex/E107 | 1 : 100 | Mw | +++ | +++ | Ki-67 | DAKO/MIB1 | 1 : 100 | Mw | + | + | MSH-2 | Genetex/EPR3943 | 1 : 100 | Mw | +++ | +++ |
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Neg: negative, +: weak, ++: moderate, +++: strong immunoexpression, and Mw: microwave.
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