Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools
Table 2
(a) CDC- and AMS-ELISA-based pretransplant crossmatch results of the 43-year-old recipient with her completely HLA-matched cadaveric kidney donor. (b) Corresponding analyses for anti-HLA antibody screening/identification.
(a)
Serum sample
CDC-CM (#)
AMS-ELISA-CM
PBL
T-cell
B-cell
Class I
Class II
10/2009
6
6
8
neg.
neg.
10/2010
6/8
6
8
neg.
neg.
neg.: negative; pos.: positive; #: NIH score system of the standard CDC-based crossmatch as a percent of positive/dead (red coloured) cells (%) as shown in Table 1(a).
(b)
Serum sample
Screening ELISA/DynaChip (Ø)
CDC cell tray (PBL)
Class I
Class II
Class I / II
07/2009
neg. (Ø)
neg. (Ø)
n.d.
10/2009
neg. (Ø)
neg. (Ø)
pos. (68% PRA)
01/2010
neg.
neg.
n.d.
04/2010
neg.
neg.
n.d.
07/2010
neg.
neg.
n.d.
10/2010
neg. (Ø)
neg. (Ø)
pos. (61% PRA)
neg.: negative; pos.: positive; n.d.: not done; the CDC-based cell tray analysis was performed during the autumnal screening (i.e., once a year in accordance with the guidelines of the European Federation of Immunogenetics (EFI)) using the samples taken in October 2009 and October 2010; Ø: additionally performed DynaChip analyses in all cases exhibiting negative results in accordance with the screening ELISA.
