The LOX-1 Scavenger Receptor and Its Implications in the Treatment of Vascular Disease
Figure 1
Internalisation of OxLDL. Epithelial HeLa cells transiently expressing a LOX-1-FLAG protein (green) were incubated with 10 g/mL DiI-OxLDL (red) for 5 mins at 37°C (pulse) to allow binding (a), and chased for a further 55 mins to allow internalization (b) before fixation. Nuclei were stained with DAPI (blue). Specimens were visualized using a deconvolution microscope using previously described procedures [26].