426369.fig.002a
(a)
426369.fig.002b
(b)
426369.fig.002c
(c)
426369.fig.002d
(d)
426369.fig.002e
(e)
426369.fig.002f
(f)
426369.fig.002g
(g)
426369.fig.002h
(h)
426369.fig.002i
(i)
Figure 2: Colocalization of actin, labeled by antibody against fragment of C-terminal domain with metaphase chromosomes. (a–c) Colocalization of intact metaphase plate with actin; (a) fluorescent staining with antibody; (b) DNA staining by TO-PRO-3; (c) merged image, bar −20 μm. (d–f) Colocalization of single chromosomes in destructed metaphase plate of squashed oocytes with actin: (d) actin staining with antibody; (e) DNA staining by TO-PRO-3; (f) merged image; arrow—centromere region where colocalization is absent, bar −8 μm. (g–i) After latrunculin B treatment, the colocalization of actin with chromosomes was preserved; (g) fluorescent staining with antibody; (h) DNA staining by TO-PRO-3; (i) merged image, bar −10 μm.