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Disease Markers
Volume 26 (2009), Issue 1, Pages 27-34
http://dx.doi.org/10.3233/DMA-2009-0601

Clinicopathological Significance of microRNA-31, -143 and -145 Expression in Colorectal Cancer

Chao-Jie Wang,1 Zong-Guang Zhou,1 Ling Wang,1 Lie Yang,1 Bin Zhou,1 Jun Gu,1 Hong-Ying Chen,1 and Xiao-Feng Sun1,2

1Institute of Digestive Surgery and Department of Colorectal Surgery, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
2Department of Oncology, Institute of Clinical and Experimental Medicine, University of Linköping, Linköping, Sweden

Received 23 February 2009; Accepted 23 February 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We are just beginning to understand how microRNAs (miRNAs) are involved in tumor-related processes in humans. Applying real-time RT-PCR, we investigated the miR-31, miR-143 and miR-145 expression in 98 primary CRC specimens, along with the corresponding normal mucosa specimens, and analyze the relationship of their expression with clinicopathological features. Our results showed the miR-31 expression was up-regulated in CRC compared to normal mucosa (p = 0.001). Furthermore, miR-31 expression was positively related to advanced TNM stage (p = 0.026) and deeper invasion of tumors (p = 0.024). MiR-145 was down-regulated in both colon (p = 0.001) and rectal (p = 0.012) cancer. MiR-143 was only down-regulated in colon cancer (p = 0.023) but not in rectal cancer (p = 0.351). There was no relationship of miR-143 and miR-145 expression with other clinicopathological features (p > 0.05), except that the miR-145 expression was related to cancer site (p = 0.03). In conclusion, the miR-31 overexpression may be involved in the development and progression of CRC. The miR-143 and miR-145 may play a certain role in the development of colon and/or rectal cancers but not in progression of the disease.